経口および吸入による水素分子の同時摂取がげっ歯類のシグナル伝達経路を相加的に抑制する
This rodent study examined how two distinct H2 delivery routes—inhalation of H2-containing air (HCA) and oral intake of H2-rich water (HRW)—affect tissue H2 concentrations and downstream signaling. Oral HRW produced a rapid but transient rise in H2 in the liver and atrial blood, whereas arterial blood and kidney concentrations reached only one-tenth of hepatic levels. HCA inhalation, by contrast, elevated H2 uniformly in both atrial and arterial compartments. DNA microarray profiling of normal mouse liver, analyzed with Ingenuity Pathway Analysis, revealed that H2 downregulated NF-κB-regulated gene sets. Western blotting confirmed attenuation of ERK, p38 MAPK, and NF-κB signaling. Crucially, concurrent administration of HRW and HCA produced stronger suppression of these pathways across systemic organs than either route alone, indicating an additive interaction. The findings suggest that H2 operates through both dose-dependent mechanisms and a broader molecular network, and that combining delivery routes may maximize biological impact.
H2 attenuates phosphorylation of ERK and p38 MAPK and reduces NF-κB-regulated gene expression, thereby exerting anti-inflammatory effects. Simultaneous oral and inhalational delivery produces additive suppression of these signaling cascades beyond what either route achieves alone.
This study combines multiple delivery routes. As a general principle, the most efficient route for routine hydrogen intake is inhalation. Inhalation carries explosion risk (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
See also:
https://h2-papers.org/en/papers/25707580