分子状水素の検出を利用した細菌検出法の開発
A detection system for bacteria was constructed and evaluated using members of the Enterobacteriaceae family, exploiting the timing of microbial hydrogen gas production. The apparatus comprised a platinum working electrode paired with a reference electrode, a buffer amplifier, and a strip-chart recorder that captured cathodic voltage shifts corresponding to H2 evolution. Response curves displayed three phases: an initial lag, a rapid potential rise, and a subsequent decline. A linear correlation was identified between initial cell density and the duration of the lag phase, with lag times spanning approximately 1 hour at 10⁶ cells/ml to 7 hours at 10⁰ cells/ml; each 10-fold reduction in inoculum extended the lag by 60–70 minutes. Mean cell density at the moment of detectable hydrogen was approximately 10⁶/ml. These findings suggest that electrochemical hydrogen sensing could serve as a rapid screening approach for coliform bacteria and other gas-producing microorganisms in clinical, food, and environmental specimens.
Electrochemical detection of H2 gas produced by Enterobacteriaceae at a platinum electrode enables bacterial quantification through the linear relationship between inoculum density and the lag time preceding measurable hydrogen evolution.
This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).
See also:
https://h2-papers.org/en/papers/4598228