水素富化生理食塩水がラットのL-アルギニン誘発急性膵炎に及ぼす保護効果
This animal study examined the effects of intravenously administered hydrogen-rich saline (>0.6 mM, 6 ml/kg) on acute pancreatitis (AP) induced in Sprague-Dawley rats by two intraperitoneal injections of L-arginine (250 mg/100 g body weight, 1-hour interval). Hydrogen-rich saline was delivered via tail vein 15 minutes after each L-arginine injection. Compared with saline controls, hydrogen-rich saline significantly reduced serum amylase activity, pancreatic edema, malondialdehyde levels, and myeloperoxidase activity, indicating suppression of lipid peroxidation and neutrophil infiltration. TUNEL staining revealed decreased apoptosis in pancreatic acinar cells, while immunohistochemistry showed enhanced PCNA expression and reduced NF-κB activation. These findings suggest that molecular hydrogen confers protection against L-arginine-induced AP through inhibition of oxidative stress, apoptosis, and NF-κB signaling, alongside promotion of acinar cell proliferation.
Molecular hydrogen scavenges hydroxyl radicals, thereby reducing oxidative stress and lipid peroxidation. This suppresses NF-κB activation and acinar cell apoptosis while promoting cell proliferation, collectively attenuating pancreatic injury severity.
Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).
See also:
https://h2-papers.org/en/papers/20138831