UV-A照射下における水素水処理がラディッシュスプラウト胚軸のアントシアニン生合成に与える影響のトランスクリプトーム解析
RNA sequencing was applied to radish sprout hypocotyls subjected to hydrogen-rich water (HRW) treatment under UV-A irradiation. Four cDNA libraries were constructed, yielding 14,564 differentially expressed genes (DEGs). GO and KEGG pathway analyses linked these DEGs to light signal perception and transduction, starch and sucrose metabolism, photosynthesis, nitrogen metabolism, and secondary metabolite biosynthesis. The MYB-bHLH-WD40 transcription factor complex was identified as the predominant regulatory unit governing anthocyanin biosynthesis, with transcript levels correlating with measured anthocyanin concentrations. Additional transcription factors—including NAC, bZIP, and TCP families—were implicated in HRW-promoted anthocyanin accumulation. Plant hormone signaling cascades, MAPK pathways, and calcium signaling were also indicated as potential mediators. Expression patterns of 16 selected genes were validated by qRT-PCR, providing a molecular framework for understanding how HRW enhances anthocyanin production under UV-A in radish sprouts.
HRW upregulates the MYB-bHLH-WD40 transcription factor complex and activates plant hormone signaling, MAPK cascades, and calcium signaling pathways, collectively enhancing anthocyanin biosynthesis in radish sprouts exposed to UV-A.
This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).
See also:
https://h2-papers.org/en/papers/30305047