水素水処理による低温ストレス応答の防御機構：トランスクリプトームとメタボノミクスの統合解析
Cold stress tolerance is a limiting factor in certain plant cultivation industries. This study employed combined metabolome and transcriptome profiling to investigate how 75% hydrogen-rich water (HRW) modulates plant defense responses under cold stress conditions. HRW application reduced stomatal density, enhanced photosynthetic efficiency, and improved resistance-related physiological parameters including Pn, Cond, MDA, and SOD. A total of 7,883 differentially expressed genes (DEGs) and 439 differentially expressed metabolites (DEMs) were identified, with DEGs predominantly associated with phenylpropanoid, isoflavonoid, monoterpenoid, and flavonoid biosynthesis pathways. Weighted gene co-expression network analysis (WGCNA) revealed a gene module strongly correlated with total antioxidant capacity and transpiration rate. Cold stress upregulated PAL, CHS, COMT, CCR, and AtBG1, leading to accumulation of coniferyl alcohol and eriodictyol; HRW treatment attenuated these elevations. The findings identify candidate genes for flavonoid biosynthesis relevant to improving cold tolerance via molecular breeding.
Cold stress upregulates PAL, CHS, COMT, CCR, and AtBG1 expression, causing accumulation of coniferyl alcohol and eriodictyol. HRW at 75% concentration modulates phenylpropanoid and flavonoid biosynthesis pathways, attenuating these metabolite elevations and improving antioxidant capacity and photosynthetic function.
Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
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https://h2-papers.org/en/papers/35812920