水素富化溶媒法によるサクラソウ（Primula veris L.）花からのフェノール類・色素・糖・有機酸・ビタミンCの抽出効率向上
This study examined how dissolving molecular hydrogen into water, ethanol, and methanol affects the extraction of bioactive compounds from wild cowslip (Primula veris L.) flowers. Hydrogen-rich methanol yielded the highest total phenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity across four assays (FRAP, DPPH, ABTS, metal chelation), followed by hydrogen-rich ethanol, with pure solvents performing least effectively. Across all solvent types, hydrogen enrichment significantly elevated TPC, TFC, and antioxidant indices, along with chlorophyll, lycopene, and β-carotene levels. HPLC-DAD/RID quantification revealed that catechin, epicatechin, rutin, quercetin, tartaric acid, succinic acid, maltose, glucose, fructose, and ascorbic acid concentrations rose by 10–90% compared with non-enriched solvents. This represents the first documented evidence that hydrogen-rich solvents enhance the co-extraction of organic acids, carotenoid pigments, and vitamin C from medicinal plant material, suggesting a sustainable and efficient approach to recovering diverse phytochemicals.
Dissolved molecular hydrogen is proposed to alter the physicochemical properties of the solvent, improving its ability to solubilize and extract phenolic compounds, organic acids, pigments, and ascorbic acid from plant cell matrices.
This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).
See also:
https://h2-papers.org/en/papers/39305640