プロバイオティクス生物水素マイクロカプセルによる腫瘍微小環境内での還元ストレス誘導と抗腫瘍効果の増強
Disruption of redox balance critically impairs cellular metabolism. This study developed probiotic biohydrogen microcapsules (PBMCs) by encapsulating Enterobacter aerogenes within gel-based microcapsules, enabling continuous H2 production inside the tumor microenvironment. PBMCs suppressed proliferation across eight tumor cell lines, including drug-resistant variants, and elevated the GSH/GSSG ratio in 4T1 cells, confirming reductive stress induction. Significant antitumor activity was demonstrated in breast cancer, melanoma, and liver cancer models. Mechanistic analysis identified inhibition of the PI3K-AKT pathway and activation of the MAPK pathway as drivers of cell cycle arrest and apoptosis. When combined with chemotherapeutic agents, PBMCs robustly inhibited preinvasive carcinoma growth and associated pulmonary metastasis, establishing in situ H2 generation as a strategy for manipulating reductive stress to increase tumor susceptibility.
Sustained H2 release from PBMCs elevates the GSH/GSSG ratio, inducing reductive stress. Concurrent inhibition of the PI3K-AKT pathway and activation of the MAPK pathway drive tumor cell cycle arrest and apoptosis.
This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).
See also:
https://h2-papers.org/en/papers/39426122