間葉系幹細胞の有効性向上における分子状水素と低温大気圧プラズマの相乗的抗酸化効果
Mesenchymal stem cells (MSCs) hold considerable promise in regenerative medicine for tissue reconstruction and immune modulation, yet their efficacy is frequently compromised by oxidative stress, immune rejection, and poor engraftment. This review examines how molecular hydrogen (H₂) and cold atmospheric plasma (CAP) may serve as adjunctive approaches to bolster MSC performance. H₂ scavenges reactive oxygen species and activates the Nrf2 signaling pathway, thereby supporting MSC survival. CAP, as a controlled source of reactive oxygen and nitrogen species, modulates cellular redox status to facilitate MSC adaptation, migration, and differentiation. When applied together, H₂ and CAP may create a microenvironment conducive to MSC engraftment and proliferation, with potential benefits across wound healing, neuroprotection, and ischemic conditions. The review also addresses optimal administration routes and dosing considerations for combined H₂ and CAP protocols, and identifies molecular targets relevant to future clinical development.
H₂ scavenges reactive oxygen species and upregulates the Nrf2 pathway to enhance MSC survival, while CAP modulates intracellular redox balance to support MSC migration and differentiation. Their combined action is proposed to create a favorable microenvironment for MSC engraftment and function.
This study combines multiple delivery routes. As a general principle, the most efficient route for routine hydrogen intake is inhalation. Inhalation carries explosion risk (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
See also:
https://h2-papers.org/en/papers/39765910