水素飽和アルカリ電解水がヒラメ筋廃用性萎縮に及ぼす影響：酸化ストレス指標を用いた検討
Female Wistar rats subjected to 3-week hindlimb unloading were allocated to four groups receiving purified water, alkaline electrolyzed water, or hydrogen-saturated alkaline electrolyzed water (HSW), alongside unloaded controls. DNA oxidation marker 8-OHdG rose approximately 149% and 145% above control in the purified-water and alkaline-water groups, respectively, whereas the HSW group showed a smaller, non-significant increase of about 95%. Lipid peroxidation (MDA) did not differ significantly across groups. SOD-like activity was markedly elevated in unloaded animals, suggesting superoxide accumulation; the HSW group showed a numerically lower elevation (169% of control) compared with the other unloaded groups, but the difference lacked statistical significance. Gastrocnemius wet-weight loss was 13% and 15% in the purified-water and alkaline-water groups, respectively, versus only 7% in the HSW group, with the HSW group showing significantly greater muscle mass than the alkaline-water group. Overall, continuous HSW consumption during hindlimb unloading did not achieve statistically robust attenuation of oxidative stress or muscle atrophy under this experimental protocol.
Hindlimb unloading elevates superoxide production in gastrocnemius muscle, increasing 8-OHdG-mediated DNA oxidation; dissolved molecular hydrogen may partially scavenge superoxide, modestly limiting oxidative damage and muscle mass loss.
Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
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https://h2-papers.org/en/papers/21963827