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Hydrogen-rich water regulates effects of ROS balance on morphology, growth and secondary metabolism via glutathione peroxidase in Ganoderma lucidum.

水素水がグルタチオンペルオキシダーゼを介してGanoderma lucidumの形態・成長・二次代謝に与える影響

in vitro study in vitro positive 5%

Abstract

Using acetic acid (HAc) as an oxidative stressor in the medicinal fungus Ganoderma lucidum, this study examined how 5% hydrogen-rich water (HRW) influences ROS levels, mycelial morphology, growth, and secondary metabolite biosynthesis. HRW administration reduced ROS accumulation, preserved biomass and polar mycelial growth, and suppressed secondary metabolism under HAc-induced stress. These effects were contingent on restoration of the glutathione system, as GPX-deficient strains and wild-type strains treated with the GPX inhibitor mercaptosuccinic acid showed no response to HRW. Conversely, GPX-overexpressing strains displayed enhanced resistance to HAc-induced oxidative stress. The findings indicate that glutathione peroxidase is an essential mediator of HRW's regulatory actions on fungal physiology and secondary metabolism.

Mechanism

HRW restores the glutathione system through glutathione peroxidase (GPX), thereby suppressing HAc-induced ROS overproduction and regulating mycelial morphology, growth, and secondary metabolite biosynthesis in G. lucidum.

Bibliographic

Authors
Ren A, Liu R, Miao Z, Zhang XQ, Cao PF, Chen TX, et al.
Journal
Environ Microbiol
Year
2017
PMID
27554678
DOI
10.1111/1462-2920.13498

Tags

Delivery:水素水経口投与 Mechanism:抗酸化酵素 グルタチオン ヒドロキシルラジカル消去 酸化ストレス 活性酸素種

Delivery context

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

Safety notes

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

See also:

Cite as: H2 Papers — PMID 27554678. https://h2-papers.org/en/papers/27554678
Source: PubMed PMID 27554678