水素富化保存液による冷虚血下ラット肺移植における虚血再灌流障害の軽減効果
Using an orthotopic left lung transplantation model in Lewis rats, this study examined whether a hydrogen-rich preservation solution (>1.0 ppm H2) could mitigate ischemia-reperfusion injury following 6-hour cold ischemia. Animals were assigned to four groups: non-transplant, minimum ischemia (<30 minutes), cold ischemia, and cold ischemia with hydrogen-rich solution. After 2-hour reperfusion, the hydrogen-rich group demonstrated significantly improved dynamic compliance, oxygenation, and wet-to-dry weight ratios relative to the cold ischemia group. Histological examination revealed reduced perivascular edema, and molecular analyses showed lower interleukin-1β mRNA expression and decreased 8-hydroxydeoxyguanosine levels, indicating attenuation of both oxidative DNA damage and inflammatory signaling. These findings suggest that hydrogen-rich preservation solutions confer pulmonary protection through antioxidant and anti-inflammatory mechanisms during cold ischemic storage prior to transplantation.
Hydrogen-rich solution reduces oxidative DNA damage by lowering 8-hydroxydeoxyguanosine levels and suppresses inflammatory signaling via decreased interleukin-1β mRNA expression, collectively attenuating pulmonary ischemia-reperfusion injury during cold ischemic preservation.
Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).
See also:
https://h2-papers.org/en/papers/31780065