水素水がDSS誘発慢性潰瘍性大腸炎マウスにおける炎症・酸化ストレス・腸内フローラ異常に与える影響
A chronic ulcerative colitis (UC) model was established in male C57BL/6 mice (7 weeks old) using multiple cycles of dextran sulfate sodium (DSS) administration. Animals received hydrogen-rich water (HRW, 0.8 ppm) daily throughout the experiment. Compared with DSS-only controls, HRW-treated mice showed partial improvement in colitis symptoms and histopathological findings, along with a significant increase in glutathione (GSH) concentration and a reduction in TNF-α levels. Notably, the relative abundance of Enterococcus faecalis, Clostridium perfringens, and Bacteroides fragilis was significantly suppressed, approaching levels observed in healthy controls. Microarray analysis identified 252 differentially expressed genes following HRW administration, of which 17 were inflammation-related, including 9 interferon-stimulated genes (ISGs). These findings suggest that HRW partially modulates oxidative stress, inflammatory signaling, and gut microbiota composition in chronic UC.
HRW elevated glutathione levels and reduced TNF-α, suppressing oxidative stress and inflammatory signaling. Microarray data indicated altered expression of 17 inflammation-related genes including 9 interferon-stimulated genes, alongside inhibition of pathogenic gut bacteria such as Enterococcus faecalis and Clostridium perfringens.
Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
See also:
https://h2-papers.org/en/papers/34784538