水素水摂取による子宮内膜癌異種移植モデルにおける差次的発現タンパク質の同定とバイオマーカーの探索
Using a xenograft mouse model of endometrial cancer (EC), this study applied tandem mass tag (TMT) labeling combined with LC-MS/MS to compare protein expression profiles between hydrogen-rich water (HRW)-treated and purified water-treated groups. Eleven differentially expressed proteins were detected: Gatad1, Ttyh3, Nek4, Dyrk2, and Gimap1 were elevated, whereas SP1, Msl1, Plekha7, Dtwd2, MSRA, and KRAS were reduced in the HRW group. Bioinformatics analysis linked these proteins to pathways including endocrine resistance, estrogen signaling, and choline metabolism in cancer. Protein interaction network analysis revealed that both KRAS and MSRA interact with YWHAE. Immunohistochemical staining confirmed strong expression of KRAS, YWHAE, and SP1 in atypical hyperplasia and EC tissues, while MSRA showed weak expression. Collectively, these four proteins—KRAS, YWHAE, SP1, and MSRA—are proposed as candidate biomarkers for assessing EC prognosis.
HRW consumption altered expression of tumor-associated proteins including KRAS, SP1, and MSRA, with pathway enrichment in estrogen signaling and endocrine resistance, suggesting modulation of these oncogenic networks may underlie the observed suppression of endometrial tumor growth.
Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
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https://h2-papers.org/en/papers/35116456