同軸繊維ドレッシングによるNO/H2逐次放出を利用した糖尿病感染創傷の抗菌・治癒促進機能の時系列制御
A coaxial fibrous wound dressing capable of temporally programmed release of nitric oxide (NO) followed by molecular hydrogen (H2) was developed to address the complex pathology of infected diabetic wounds. The outer fiber layer, physically loaded with an NO donor, released NO at concentrations exceeding 170 μM during the initial phase, achieving bacterial eradication rates of 99.99% against Staphylococcus aureus and 99.04% against Escherichia coli, thereby facilitating resolution of the inflammatory phase. As the outer layer degraded, the inner layer became exposed, enabling near-infrared (NIR)-triggered hydrolysis of an encapsulated H2 donor for on-demand H2 release. The H2 promoted tissue repair through reactive oxygen species (ROS) scavenging and macrophage M2 polarization, as confirmed by in vitro cell assays. In a full-thickness infected skin defect model in diabetic mice, the sequential NO/H2 dressing significantly accelerated wound closure by combining rapid bactericidal action with subsequent regenerative H2 signaling.
The outer fiber layer releases NO rapidly to eliminate bacteria and shorten the inflammatory phase; subsequent structural degradation exposes the inner layer, where NIR irradiation triggers H2 donor hydrolysis, producing H2 that scavenges ROS and induces macrophage M2 polarization to support tissue regeneration.
Topical applications have localized-effect reports, but systemic hydrogen intake is most efficient via inhalation. Inhalation carries explosion risk (empirical LFL of 10%; high-concentration devices are not recommended).
See also:
https://h2-papers.org/en/papers/40504663