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Cadmium-Induced Hydrogen Accumulation Is Involved in Cadmium Tolerance in Brassica campestris by Reestablishment of Reduced Glutathione Homeostasis.

カドミウム誘導性水素蓄積はグルタチオン恒常性の再確立を通じてブラッシカ・カンペストリスのカドミウム耐性に関与する

in vitro study hydrogen-rich water positive 50%

Abstract

This study examined the physiological role of hydrogen-rich water (HRW) in Brassica campestris seedlings exposed to cadmium (Cd) stress. Both 50 μM CdCl2 and HRW at 50% saturation elevated endogenous H2 levels. HRW application reduced growth inhibition and oxidative damage caused by Cd. Seedlings receiving HRW showed greater root elongation, lower lipid peroxidation, elevated reduced glutathione (GSH) and ascorbic acid (AsA) concentrations, and higher GST and GPX enzymatic activities in roots. At the molecular level, expression of GS, GR1, and GR2 genes—suppressed by Cd, GSH, or BSO—was partially restored by HRW supplementation. These findings indicate that H2 functions as a regulator of GSH homeostasis, thereby enhancing Cd tolerance in Brassica campestris seedlings.

Mechanism

H2 restores expression of GSH biosynthesis and recycling genes (GS, GR1, GR2), thereby re-establishing reduced glutathione and ascorbic acid homeostasis and mitigating cadmium-induced oxidative stress in plant roots.

Bibliographic

Authors
Wu QF, Su N, Chen Q, Shen W, Shen Z, Xia Y, et al.
Journal
PLoS One
Year
2015
PMID
26445361
DOI
10.1371/journal.pone.0139956
PMC
PMC4596834

Tags

Disease:重金属毒性 Delivery:水素水経口投与 Mechanism:抗酸化酵素 グルタチオン 脂質過酸化 酸化ストレス 活性酸素種

Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:

Other papers on the same disease / condition

Cite as: H2 Papers — PMID 26445361. https://h2-papers.org/en/papers/26445361
Source: PubMed PMID 26445361