水素包接シリカが食道細胞の遊走およびアポトーシスに与える影響
To circumvent the strict handling requirements of hydrogen gas in clinical and laboratory settings, this study examined a solidified hydrogen-occluding silica (H-silica) capable of releasing molecular hydrogen into cell culture medium. Human esophageal squamous cell carcinoma cells (KYSE-70) and normal human esophageal epithelial cells (HEEpiCs) were exposed to varying H-silica concentrations. Proliferation was suppressed in a concentration-dependent manner in KYSE-70 cells. Apoptosis induction, assessed via Bax/Bcl-2 ratio and cleaved caspase-3 by western blot, was observed at 10, 300, 600, and 1,200 ppm in KYSE-70, while only 1,200 ppm produced a 2.4-fold increase in apoptosis in normal cells. Intracellular reactive oxygen species were measured by nitroblue tetrazolium assay. Cell migration assays demonstrated inhibition in KYSE-70 cells, and high H-silica concentrations exhibited cytotoxic effects on normal esophageal cells. These findings offer mechanistic insight into how H-silica suppresses human cancer cell behavior.
Molecular hydrogen released from H-silica reduces intracellular reactive oxygen species and promotes apoptosis in esophageal carcinoma cells through upregulation of the Bax/Bcl-2 ratio and activation of cleaved caspase-3, thereby suppressing proliferation and migration.
This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).
See also:
https://h2-papers.org/en/papers/28744359