マイコトキシン汚染飼料を与えた雌豚仔における水素水およびラクツロース経口投与に対する腸内微生物叢の生態学的応答
Twenty-four female piglets (Landrace × Large × White; mean initial body weight 7.25 ± 1.02 kg) were divided into four groups and observed over 25 days: an uncontaminated basal diet group, a mycotoxin-contaminated diet (MC) group, an MC + hydrogen-rich water (HRW) group, and an MC + lactulose (LAC) group. Mycotoxin exposure reduced hydrogen concentrations in the cecal mucosa, altered short-chain fatty acid (SCFA) profiles, and disrupted microbial community composition. HRW administration elevated hydrogen levels in the stomach and duodenum, while both HRW and LAC maintained higher colonic and cecal hydrogen concentrations compared with the MC group. Both interventions counteracted mycotoxin-associated increases in diarrhea rate and decreases in SCFA production in the colon and cecum. Denaturing gradient gel electrophoresis (DGGE) analysis indicated that HRW and LAC shifted hydrogen-utilization pathways and altered microbial diversity. Furthermore, mycotoxin-induced changes in specific bacterial populations in ileal digesta and hydrogen-utilizing bacteria in colonic digesta were reversed by both HRW and LAC supplementation.
HRW elevates luminal hydrogen concentrations in the upper gastrointestinal tract and modulates hydrogen-utilizing bacterial pathways in the cecum and colon, thereby counteracting mycotoxin-induced dysbiosis and suppression of short-chain fatty acid production.
Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
See also:
https://h2-papers.org/en/papers/29914163