DSS誘発マウス大腸炎モデルにおける水素リッチウォーターとスルファサラジンの効果比較
An acute colitis model was established in mice by adding dextran sulfate sodium (DSS) to drinking water for seven days. Animals were divided into five groups: normal, colitis control, hydrogen-rich water (HRW), sulfasalazine, and HRW plus sulfasalazine. HRW was prepared using a hydrogen-generating tablet dissolved in water and administered both ad libitum and via oral gavage (200 µL) from days 3 to 10. Both HRW and sulfasalazine significantly improved body weight, Disease Activity Index scores, mucosal integrity, crypt preservation, and spleen weight relative to untreated colitis controls. Reductions in high-sensitivity C-reactive protein and restoration of redox markers—including total thiol content, superoxide dismutase, and catalase activity—were observed in both treatment groups. HRW showed a tendency to match or exceed the efficacy of sulfasalazine, and the combination regimen demonstrated an additive trend beyond either agent alone.
HRW is proposed to suppress colonic inflammation and oxidative stress by reducing high-sensitivity CRP levels and restoring antioxidant capacity, including total thiol content, superoxide dismutase, and catalase activity.
Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
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https://h2-papers.org/en/papers/34345230