急性脳虚血再灌流傷害マウスモデルにおける水素水の認知機能改善・抗酸化・抗アポトーシス・抗炎症効果
Using a bilateral common carotid artery occlusion model in C57BL/6 mice, this study evaluated the effects of orally administered hydrogen-rich water on cerebral ischemia-reperfusion injury. Behavioral assessments (open field and novel object recognition tests) demonstrated improved cognitive performance and reduced anxiety in hydrogen-treated animals. Histological analysis showed fewer pyknotic cells in the brains of treated mice. Biochemical assays indicated elevated total antioxidant capacity and reduced thiobarbituric acid reactive substance levels, reflecting antioxidative activity. Immunofluorescence microscopy revealed decreased apoptosis in the striatum, cerebral cortex, and hippocampus. Western blotting confirmed reductions in Bax and TNF-α protein levels, while cytokine profiling showed significant differences in IL-2 and IL-10 concentrations between treated and untreated groups, suggesting modulation of inflammatory signaling.
Hydrogen-rich water is proposed to scavenge reactive oxygen species, thereby reducing oxidative stress. Downstream effects include suppression of Bax-mediated apoptosis and modulation of inflammatory cytokines (IL-2, IL-10, TNF-α) in ischemia-reperfusion-injured brain tissue.
Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
See also:
https://h2-papers.org/en/papers/34746315