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The Effect of Molecular Hydrogen on Functional States of Erythrocytes in Rats with Simulated Chronic Heart Failure.

慢性心不全モデルラットにおける赤血球機能状態への分子状水素の影響

animal study inhalation positive

Abstract

Using a rat model of chronic heart failure (CHF), this study examined how H2 gas inhalation influences erythrocyte functional states. Measured parameters included lipid peroxidation markers, antioxidant capacity, electrophoretic mobility of erythrocytes (EPM), aggregation, ATP and 2,3-diphosphoglyceric acid (2,3-DPG) levels, and hematological indices. Both single and repeated H2 inhalation sessions were associated with elevated EPM and reduced erythrocyte aggregation, with more pronounced changes observed after multiple exposures. Lipid peroxidation dynamics in erythrocytes paralleled oxidative changes in blood plasma under both exposure conditions. The findings suggest that the antioxidant properties of molecular hydrogen may underlie its metabolic effects, ultimately improving microcirculation and the oxygen-carrying capacity of blood in CHF conditions.

Mechanism

H2 inhalation is proposed to suppress erythrocyte lipid peroxidation via antioxidant activity, leading to increased electrophoretic mobility and reduced aggregation, thereby enhancing microcirculation and blood oxygen transport in CHF.

Bibliographic

Authors
Deryugina AV, Danilova DA, Pichugin VV, Brichkin YD
Journal
Life (Basel)
Year
2023 (2023-02-02)
PMID
36836774
DOI
10.3390/life13020418
PMC
PMC9960520

Tags

Disease:myocardial infarction (28) Delivery:inhalation delivery (196) Mechanism:antioxidant enzymes (423) anti-inflammatory (743) lipid peroxidation (238) oxidative stress (899) reactive oxygen species (774)

Delivery context

In air, molecular hydrogen is reported to be combustible across approximately **4% (LFL, lower flammability limit) to 75% (UFL, upper flammability limit)**. Among high-concentration hydrogen inhalers, 66% output sits inside this range, and even pure-hydrogen (100%) output forms a 4–75% concentration-gradient layer at the device–air boundary (the UFL 75% paradox). Engineering principle would therefore call for operation below LFL (the classical 4%); that figure, however, was measured under closed, pre-mixed, static conditions. For the open, dynamic inhalation environment, the empirical value reported in the literature is **10%**, which is the figure referenced in practice as the operating ceiling. The 66% / 100% output devices are recorded in the Japanese Consumer Affairs Agency accident-information database, and from these considerations are not recommended.

→ Evidence by delivery route

Safety notes

In air, molecular hydrogen is reported to be combustible across approximately **4% (LFL, lower flammability limit) to 75% (UFL, upper flammability limit)**. Among high-concentration hydrogen inhalers, 66% output sits inside this range, and even pure-hydrogen (100%) output forms a 4–75% concentration-gradient layer at the device–air boundary (the UFL 75% paradox). Engineering principle would therefore call for operation below LFL (the classical 4%); that figure, however, was measured under closed, pre-mixed, static conditions. For the open, dynamic inhalation environment, the empirical value reported in the literature is **10%**, which is the figure referenced in practice as the operating ceiling. The 66% / 100% output devices are recorded in the Japanese Consumer Affairs Agency accident-information database, and from these considerations are not recommended.

See also:

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