水素水投与がRT1-Db1およびRT1-Bbを標的として早発卵巣不全ラットの卵巣障害を軽減する
Using a cyclophosphamide-induced premature ovarian failure (POF) rat model, this study examined the ovarian-protective effects of hydrogen-rich water (HRW). Serum anti-Müllerian hormone (AMH) and 17-β-estradiol (E2) levels rose significantly in HRW-administered animals, while follicle-stimulating hormone (FSH) declined, alongside improved ovarian histomorphology and reduced apoptosis by TUNEL assay. Tandem mass tag (TMT)-based quantitative proteomics identified 16 candidate differentially expressed proteins through cross-comparison of POF vs. control and POF+HRW vs. POF groups. These proteins were enriched across 296 Gene Ontology terms and 36 KEGG pathways. Integration of protein-protein interaction and GeneMANIA network analyses pinpointed RT1-Db1 and RT1-Bb as the principal molecular targets underlying HRW-associated ovarian protection in POF rats.
HRW modulated the expression of MHC class II-related proteins RT1-Db1 and RT1-Bb in ovarian tissue, associated with reduced apoptosis, improved follicular histomorphology, and restoration of reproductive hormone balance (AMH, E2, FSH) in cyclophosphamide-induced POF rats.
Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).
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https://h2-papers.org/en/papers/37397014