水素放出マグネシウムハイドロゲルによる椎弓切除術後硬膜外線維症の抑制：好中球細胞外トラップ形成阻害を介したメカニズム
Epidural fibrosis following laminectomy is a major cause of failed back surgery syndrome, with neutrophil extracellular traps (NETs) playing a central role in its pathogenesis. Reactive oxygen species (ROS) drive NET formation at the surgical site. To address this, researchers engineered a sustained hydrogen-delivery system by embedding PLGA-coated biodegradable magnesium microspheres (Mg@PLGA) into a thermosensitive PLGA-PEG-PLGA hydrogel scaffold (Mg@PLGA@Gel). In vitro assessments confirmed that hydrogen generated by Mg@PLGA effectively scavenged intracellular ROS in neutrophils and suppressed NET formation while maintaining good biocompatibility. In a mouse laminectomy model, histological evaluations using H&E, Masson staining, MRI, and immunohistochemistry demonstrated that Mg@PLGA@Gel significantly reduced epidural scarring, ROS accumulation, and NET deposition at the surgical site. These findings indicate that continuous local hydrogen release from this implantable hydrogel system can mitigate post-laminectomy epidural fibrosis.
Hydrogen released from magnesium microspheres selectively neutralizes intracellular ROS in neutrophils, thereby suppressing NET formation and downstream fibrotic signaling at the laminectomy site.
This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).
See also:
https://h2-papers.org/en/papers/39260811