低酸素後処理と水素ガスの併用による単離ラット心臓の梗塞縮小効果：新たな心臓保護介入の検討
Reactive oxygen species generated during ischemia-reperfusion (I/R) are known to impair cardiac function. Using an isolated rat heart model subjected to 30 minutes of global ischemia followed by 120 minutes of reperfusion, this study evaluated the cardioprotective potential of hypoxic postconditioning (HpostC) delivered via oxygen-free Krebs-Henseleit buffer (KHB), as well as a combined intervention using H2-saturated oxygen-free KHB (H2+HpostC). Four alternating cycles of 1-minute oxygen-free and 1-minute normal KHB perfusion were applied at reperfusion onset. Endpoints included infarct size as a percentage of area at risk (IS/AR), recovery of cardiac function, and incidence of reperfusion arrhythmias. HpostC alone significantly reduced IS/AR versus non-conditioned controls. The H2+HpostC combination produced a further reduction in IS/AR, suppressed severe arrhythmias, and significantly restored post-I/R heart function, indicating that molecular hydrogen augments the protective effect of hypoxic postconditioning.
Molecular hydrogen selectively reacts with potent oxidants and diffuses freely into cells, reducing oxidative stress without disrupting normal redox metabolism, thereby augmenting the cardioprotective effect of hypoxic postconditioning during reperfusion.
Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).
See also:
https://h2-papers.org/en/papers/28350967