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Protective Effect of Hydrogen-Rich Saline on Spinal Cord Damage in Rats.

水素富化生理食塩水がラット脊髄損傷に与える保護効果

animal study injection / infusion positive

Abstract

This animal study examined the effects of intraperitoneally administered hydrogen-rich saline (HRS) on spinal cord injury (SCI) in 4-month-old male Sprague Dawley rats (n=24) divided into four groups: laminectomy-only control, SCI without treatment, HRS alone, and SCI followed by 7-day HRS administration. Spinal cord injury was induced at the T7-T10 vertebral level using the Tator and Rivlin clip compression model for 1 minute with the dura left intact. Blood samples collected on day 7 showed significantly reduced interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) concentrations in the HRS-treated SCI group compared to the untreated SCI group. Histological assessment using hematoxylin-eosin and TUNEL staining further revealed a reduction in apoptotic cell death in spinal cord tissue. These findings suggest that HRS exerts both anti-inflammatory and anti-apoptotic effects in the context of acute spinal cord compression injury.

Mechanism

Intraperitoneal HRS administration suppressed pro-inflammatory cytokines IL-6 and TNF-α in blood following spinal cord compression, and reduced apoptotic cell death in spinal tissue, indicating anti-inflammatory and anti-apoptotic mechanisms.

Bibliographic

Authors
Kayabaş M, Şahin L, Makav M, Alwazeer D, Aras L, Yiğit S, et al.
Journal
Pharmaceuticals (Basel)
Year
2023 (2023-04-01)
PMID
37111284
DOI
10.3390/ph16040527
PMC
PMC10143771

Tags

Disease:脊髄損傷 Delivery:点滴投与 Mechanism:アポトーシス抑制 炎症抑制 酸化ストレス 活性酸素種

Delivery context

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

Safety notes

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

See also:

Other papers on the same disease / condition

Cite as: H2 Papers — PMID 37111284. https://h2-papers.org/en/papers/37111284
Source: PubMed PMID 37111284